The expression of CK1 became highly significant after 21 days in the cells cultured on the mp CHT membrane, where keratinocytes grew as compact multilayers with thickness of 38–42 μm ( Figure 7 c,d) as an indication of their terminal differentiation.
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Nano- and Micro-Porous Chitosan Membranes for Human Epidermal Stratification and Differentiation.
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