A facile, versatile hydrogel bioink for 3D bioprinting benefits long-term subaqueous fidelity, cell viability and proliferation.

As the culturing continued, the proportion of calcein-AM + cells in GEL–ALG/CMC/AHA constructs showed an increasing trend, while in GEL–ALG constructs it exhibited a contrary tendency ( Fig. 7F ).

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The difference was that the cell growth in GEL–ALG/CMC/AHA was still in a plateau stage from days 17 to 29, while the cell growth in GEL–ALG showed a decreasing trend after day 17 ( Fig. 8A ).

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