The results of secreted TIMP-2 expression in the supernatants showed a decreasing trend when SCs were cocultured with IECs, and the secreted TIMP-2 expression level was significantly lower in the supernatants of the SC and IEC coculture system than without coculture (Fig. 3 f).
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Schwann cells promote prevascularization and osteogenesis of tissue-engineered bone via bone marrow mesenchymal stem cell-derived endothelial cells.
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The WB results showed that the expression levels of nestin, Flt1, and Kdr were significantly higher in IECs cocultured with SCs than in IECs cultured without SCs and revealed an increasing trend as the time in coculture increased (Fig. 3 d).