Indeed, normalized quantitation of DDX21 immunofluorescence confirmed a highly significant reduction in overall average expression per cell (encompassing both microinjected and non-microinjected cell clones) in the Ddx21 siRNA treatment group versus the NTC control ( figure 3 c – e ; electronic supplementary material, figure S4a–c).
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DDX21 is a p38-MAPK-sensitive nucleolar protein necessary for mouse preimplantation embryo development and cell-fate specification.
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