Analysis of SARS-CoV-2 reverse transcription-quantitative polymerase chain reaction cycle threshold values vis-à-vis anti-SARS-CoV-2 antibodies from a high incidence region.

Ct values and interval between onset of symptoms and sample collection Statistically highly significant ( P <0.001) correlations of only low to medium effect size could be shown between the Ct value of all 3 genes measured with Assay 1 and the amount of days since onset of symptoms (Spearman correlation coefficients: E gene 0.343; N gene 0.363; RdRp gene: 0.344) whereas for Assay 2 the same effect could be seen only for the E gene (Spearman correlation coefficient: 0.332; see Figure 5 ).

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