highly significantp < 0.000001
The heart, which had the lowest spirochete load, was set as the reference; the spirochete load was higher in the bladder (5.2x), ankle joint (5.4x), dorsal skin (25.7x), right ear (122.2x), and left ear (147.7x), and these differences among mouse organs were highly significant (Fig. 1 ; p < 0.000001; see section 5 in the ESM for the complete analysis).