AID deamination activity is also detected on the NTS in the 176 bp region of E2A when there is no transcription through the substrate ( Figure 4B ), but this occurs at a somewhat lower rate than what is observed on the substrate with transcription (borderline significant with p = 6.4 × 10 −2 ).
In subsequent studies, we showed that the CG motif was highly significant for being the preferred site of activation-induced deaminase (AID) action for the genes mentioned earlier and for additional B cell oncogenes, including CRLF2 and MALT1 , within their <600 bp fragile zones ( Cui et al., 2013 ; Greisman et al., 2012 ; Pannunzio and Lieber, 2017 ; Tsai et al., 2008 , 2010a , 2010b ).