Cell line development for continuous high cell density biomanufacturing: Exploiting hypoxia for improved productivity.

When comparing the mean fluorescence of DG44-5HRE-VEGF cells cultured under normoxic and hypoxic conditions, a highly significant and nearly 10-fold induction of the d2GFP signal could be detected after 140 h, while no significant induction was observed for control cell lines ( Fig. 3 C).

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