An open-source pipeline for analysing changes in microglial morphology.

Of note, abuse of the inflammation index, by using control and HFD microglia as a training dataset to create an index that is optimally sensitive to the test data, produces a highly significant difference in a statistical test of control (mean ± s.d. of −0.73 ± 1.74) versus HFD (0.55 ± 1.89) conditions ( p = 0.0027; linear mixed model with the animal ID specified as a random intercept).

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