Wheat with greatly reduced accumulation of free asparagine in the grain, produced by CRISPR/Cas9 editing of asparagine synthetase gene TaASN2.

For the separate lines, the difference in free asparagine concentrations between both triple null lines, 23 and 59, and wildtype was shown to be significant ( p = 0.0402 for line 23 and p = 0.0122 for line 59, t ‐test) while that for the A genome null line, 178, was marginally significant ( p = 0.0919, t ‐test).

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