Evaluation of Aqueous Biphasic Electrophoresis System Based on Halide-Free Ionic Liquids for Direct Recovery of Keratinase.

Moreover, the keratinase recovery in the biphasic system comprised of 15.0% ( w/w ) [EMIM][ESO 4 ] was improved with the increment of carbonate concentration from 17.5% ( w/w ) to 20.0% ( w/w ), followed by a decreasing trend when the carbonate concentration was further increased to 22.5% ( w/w ) and 25.0% ( w/w ).

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The finding was in accordance with the aforementioned statement whereby an increasing trend was demonstrated as the concentration of crude feedstock increased from 10% ( w/w ) (K e = 3.58 ± 0.43, Y T = 71.48% ± 2.41, PF T = 2.02 ± 0.08) to 15% ( w / w ) (K e = 6.15 ± 0.52, Y T = 81.19% ± 1.39, PF T = 2.49 ± 0.09).

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