Deciphering the low abundance microbiota of presumed aseptic hip and knee implants.

A positive control-associated artifact is highly significant Some samples contained unusually large proportions of Staphylococcus sp. and Escherichia-Shigella sp. 16S rRNA sequencing often cannot identify species, but the Staphylococcus sp. and Escherichia-Shigella sp. identified in the affected samples are almost certainly the Staphylococcus aureus Newman and Escherichia coli DH5α positive controls given their abundance and pattern, as well as the fact that they were the only detectable Staphylococcus sp. and Escherichia-Shigella sp.

also in 132,142 other papers