an increasing trendP > 0.05
Results Experiment 1 In this experiment, buffaloes treated with GPG showed a higher estrus rate (96.1%; P < 0.05) with an increasing trend in ovulation (84.6%; P > 0.05), and pregnancy rate (30.8%; P > 0.05) with lower silent estrus (3.8%; P < 0.05) and follicular cyst (3.8%; P < 0.05) rates compared to PmPG, PGPE, and P 4 PmPG treated buffaloes.