The ageing-related proteins, p16 INK4A , p21 Waf1/Cip1 , p- γ H2AX, and β-galactosidase, presented a decreasing trend in the testicular tissue in the ageing model compared to the control group; this trend was reversed by LBSO treatment (all p < 0.001) ( Figure 1(C–G) ).
LBSO alleviated oxidative stress-induced testis injury of ageing rats Compared to the ageing model of rats, the oxidation-related proteins, such as HO-1 ( p < 0.05) ( Figure 2(B) ), NRF2 ( p < 0.05) ( Figure 2(C) ), SOD-1 ( p < 0.05) ( Figure 2(D) ), and SOD-2 ( p < 0.05) ( Figure 2(E) ) presented an increasing trend in the testicular tissue of the LBSO group as compared, thereby presenting the effects of LBSO on antioxidative stress in the testis of ageing rats.