highly significantP -value < 10 −3
The SNPs that did not match the following criteria were removed before performing population diversity analyses: (i) SNPs with call rates less than 90%, (ii) SNPs with minor allele frequencies less than 5%, (iii) SNPs with more than 1% missing genotypes, and (iv) SNPs displaying highly significant deviations ( P -value < 10 −3 ) from the Hardy-Weinberg equilibrium ( Amaral, Pavão & Gama, 2020 ).