Acute social isolation and regrouping cause short- and long-term molecular changes in the rat medial amygdala.

While there was a borderline significant interaction between encounter and condition (Mixed model ANOVA - encounter × condition: F (1,22) = 4.338, P = 0.049), post hoc analysis revealed that OEE rescued SRM in acutely isolated rats, as both groups showed a significantly reduced investigation time in E2 compared to E1 ( post hoc paired t -test - G: t 10 = 5.131, P < 0.0001; Iso 7d: t 12 = 4.836, P < 0.0001; Supplementary Fig. 2a ).

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Statistical analysis revealed a highly significant interaction between individuals (novel vs. familiar) and housing conditions (G vs.

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