Effects of sequence motifs in the yeast 3' untranslated region determined from massively parallel assays of random sequences.

cerevisiae proteins [ 45 ], resulted in a highly significant alignment to Hrp1 for the enriched N50-C motif containing UAUAUA, as well as two other enrichment-associated motifs; the other motifs did not yield significant matches to these four RNA-binding proteins (Additional File 1 : Fig.

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Searching for motifs associated with either high or low enrichment (> 2.5σ away the mean), and using a randomly sampled set of library sequences of the same size as the background, we found that by far the most significant and frequently occurring motif was an N50-C motif associated with enrichment and containing a UAUAUA 6-mer ( p = 1.12 × 10 −43 ); the few other motifs reported by STREME had barely significant p values (especially given the large library size) and did not occur often (Additional File 1 : Fig.

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