marginally non-significantp =0.12
Linear trends (estimating the average slopes of curves in Figure 1 ) shifted from negative after embryogenesis at 18°C to positive after embryogenesis at 22°C ( Figure 2A ), and differed significantly between 18°C and both of the other temperatures (the contrast between 20 and 22°C was marginally non-significant at p =0.12; Figure 2D ).