Tween^® Preserves Enzyme Activity and Stability in PLGA Nanoparticles.

While all Tween ® 20:enzyme solution mixtures yielded NPs with higher activity respective to those without Tween ® 20, the difference between the various mixtures was insignificant, showing only a slight trend towards higher activity correlated to a higher amount of Tween ® 20 in the initial solution, as well as the trend towards higher Tween ® 20:β-Glu molar rapports ( Figure 4 , Table 5 ).

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