Pollen quantification using metabarcoding Highly significant positive relationships between the relative abundance of sequencing reads (RRA) and relative abundance of microscopically obtained pollen concentrations were found for all studied taxa using trn L and nrITS2 ( p < 0.001 for all correlations; Fig. 3 ).
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DNA metabarcoding using nrITS2 provides highly qualitative and quantitative results for airborne pollen monitoring.
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