Excessive energy expenditure due to acute physical restraint disrupts Drosophila motivational feeding response.

If the mounting period is not long, the effects of energy depletion due to restraint-induced struggle may not be significant, however for assays where recording for longer than a few minutes is necessary, confounding effects are possible.

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In addition, we show that the consequences of the physical restraint profoundly alter aerobic activity, energy depletion, taste, and feeding behavior, and suggest that careful consideration is given to the time-sensitive nature of these highly significant effects when conducting behavioral, physiological or imaging experiments that require immobilization.

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