An ELISA has been validated and found to be simple, rapid, and accurate for detection of NME and ME in human urine, with highly significant correlations to HPLC results. 29 We evaluated a human ELISA for analysis of metanephrines in feline urine, and found acceptable performance for analysis of NME, except for dilution recovery. 26 Our aim was to verify a urine ELISA for analysis of the metanephrines NME and ME in canine urine, and to investigate potential effects of acidification, room temperature storage time, sampling time (morning or midday), and time in a clinic environment on concentrations of urine metanephrines.
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Quantification of normetanephrine in canine urine using ELISA: evaluation of factors affecting results.
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