extremely significantp < 0.0001
With a confidence value of α = 0.05, values p > 0.05 were considered statistically not significant (ns); those between 0.01 < p < 0.05 were considered significant (*); 0.001 < p < 0.01 were very significant (**); 0.0001 < p < 0.001 were extremely significant (***) as well as those p < 0.0001 (****). t-test was used to assess statistical differences between two sample means (two-tailed paired or unpaired, when appropriate).