Additionally, in the unrelated individuals ( Figure 2 ), He showed a decreasing trend in the last two periods, which was more evident in the period 2011–2019.
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Nevertheless, considering all the horses, related and unrelated (all potentially breeders), a small trend towards some decrease in heterozygosity in the last ten years was recorded which was not strong enough to generate concern at this moment in time.
The total population showed significantly different Ho and He (0.692 and 0.734, respectively), resulting to significant deviation from HWE (Mean P-val 0.001; Figure 1 ), as expected in a population including related individuals, with eight loci being highly significant P-val ( Figure 1 ).