Mammalian brain glycoproteins exhibit diminished glycan complexity compared to other tissues.

The cerebellum of female mice showed less biantennary glycans, an increase in sialylation, and an overall trend toward more complex structures compared to the males.

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We noted very few O-glycans containing both sialic acid and fucose in the brain (<2% in all regions), and simple linear regression of fucosylated vs sialylated O-glycans showed a strong and highly significant negative correlation in both O-GalNAc and O-Man glycans (Supplementary Fig. 3 ).

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