Glucocorticoid Regulates the Synthesis of Porcine Muscle Protein through m⁶A Modified Amino Acid Transporter <i>SLC7A7</i>.

Effect of GCs on the Amino Acid Transporter SLC7A7 We extracted RNA after DEX treatment for 48h in porcine skeletal muscle cells and used QPCR to determine the mRNA expression levels of relevant amino acid transporters, which revealed a highly significant downregulation of the mRNA expression levels of the amino acid transporters SLC1A3 , SLC7A7 , and SLC7A10 ( Figure S1 ).

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Finally, the 2 −ΔΔ Ct method was used for data analysis, and one-way analysis of variance was performed to determine the significance at p < 0.05 (significant) and p < 0.01 (extremely significant).

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