WAPO-A1 is the causal gene of the 7AL QTL for spikelet number per spike in wheat.

In the hexaploid BC 5 F 3 NILs, tested in small plots, we detected a 6.1% increase in SNS in the H2-NILs relative to the H1-NILs ( P = 0.0092, Fig 8E and panel G in S4 Table ) and an 8.4% increase in grain yield that was marginally significant ( P = 0.0497, Fig 8F and panel H in S4 Table ).

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Previously, a highly significant QTL affecting spikelet number per spike (SNS), grain number per spike (GNS) and grain yield was detected on chromosome arm 7AL in multiple genome-wide association studies.

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