The parameters could be reduced to only four highly significant factors: BSA concentration, impregnation method, impregnation time, and electron beam irradiation dose.
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Highly Efficient One-Step Protein Immobilization on Polymer Membranes Supported by Response Surface Methodology.
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The sentences
For the BSA concentration, the contour plots showed that a plateau of BSA GY is reached when using the 2-step impregnation method, but a slightly increasing trend for the 1-step method was observable.