highly significantp ≤ 0.01
Statistical differences were designated as significant if p -values were less than 0.05 ( # p ≤ 0.05), and highly significant if p -values were less than 0.01 ( ## p ≤ 0.01), less than 0.001 ( ### p ≤ 0.001) and less than 0.0001 ( #### p ≤ 0.0001).