RT-qPCR Detection of SARS-CoV-2: No Need for a Dedicated Reverse Transcription Step.

Int J Mol Sci · 2022 · PMC8835954 · PMID 35163227

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a possible trendno p-value reported

Results for both temperatures were similar ( Figure 9 C), although there was a possible trend for the three assays targeting the E-gene recording lower Cqs with hexamers at 30 °C.

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