trending toward significanceP = 0.07
The deletion from − 1878 bp to − 1523 bp showed a decrease of luciferase activity that was trending toward significance ( P = 0.07); when the deletion reached the − 1264 bp, the luciferase activity was significantly increased ( P < 0.01); When the deletion reached − 953 bp, the luciferase activity was significantly reduced ( P < 0.01), indicating that there are negative regulatory elements in the region from − 1264 bp to − 953 bp.