A Three-Dimensional Organoid Model of Primary Breast Cancer to Investigate the Effects of Oncolytic Virotherapy.

A mock infection containing breast cancer organoids and culture medium was defined as maximum viability (100%); 1) cultivation of MeV-SCD-infected organoids in presence of the prodrug 5-FC resulted in a highly significant drop in viability to 26% ( Figure 4D , bar 4) when compared to the negative control ( Figure 4D , bar 2; incubation only with the non-toxic prodrug 5-FC, no infections) ( p < 0.0001); 2) cultivation of MeV-SCD infected organoids in the absence of the prodrug 5-FC displayed an intrinsic oncolytic effect (without additional tumor cell-bound conversion of 5-FC into 5-FU and derivatives), which resulted in a significant drop of viability ( p < 0.5) to only 67% ( Figure 4D , bar 3) compared to 5-FC alone ( Figure 4D , bar 2).

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