Specific examples include 3-hydroxy-3-methylglutarate—which displayed an increasing trend in the ULF lavage of one subject on Day 12 (Fig. 2g ), yet the opposite trajectory in a different Day 12 subject (Fig. 2h ).
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To elucidate common trends in metabolite flux, metabolomic data (Supplementary Data 2 ) were compared by two-way ANOVA, with P ≤ 0.05 denoting significance and 0.05 < P < 0.10 highlighting a trend toward significance.