highly significantP < 0.05
The GPR15 sequences of two lineages of fishes ( Latimeria chalumnae and Lepisosteus oculatus/Scleropages formosus ) had a large nonsynonymous (d N )/synonymous (d S ) substitution rate ratio (branch site d N /d S of ω >> 1; Fig 1 A and D), which was highly significant (likelihood ratio tests [LRTs], P < 0.05; Fig 1D ).