Ample dietary fat reduced the risk of primary vesical calculi by inducing macrophages to engulf budding crystals in mice.

Immunohistochemical staining of the macrophage marker F4/80 showed that the macrophages in the HF group had a higher infiltration level and an obvious trend of aggregation in the bladder urothelium compared to those in the NF and LF groups when treated for four weeks.

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The amount of CXCL14 protein showed an increasing trend with the concentration of palmitic acid and oleic acid ( Fig. 6 A).

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