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Preparation of Highly Substituted Sulfated Alfalfa Polysaccharides and Evaluation of Their Biological Activity.

Foods · 2022 · PMC8909867 · PMID 35267371

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an increasing trendno p-value reported
At different ratios, the degree of substitution keeps an increasing trend with increasing temperature until the temperature reaches the central point, and decreases after reaching the central point.

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a decreasing trendno p-value reported
Shorter reaction times would lead to incomplete reactions, but when the reaction time was more than 2.5 h, the degree of substitution showed a decreasing trend.

also in 47,180 other papers

Quoted from the open-access full text in Europe PMC under the licence the publisher applied. The sentence is reproduced exactly as published; the emphasis is ours.