At different ratios, the degree of substitution keeps an increasing trend with increasing temperature until the temperature reaches the central point, and decreases after reaching the central point.
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Preparation of Highly Substituted Sulfated Alfalfa Polysaccharides and Evaluation of Their Biological Activity.
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The sentences
Shorter reaction times would lead to incomplete reactions, but when the reaction time was more than 2.5 h, the degree of substitution showed a decreasing trend.