Biochemical Characterization of a Human Septin Octamer.

Front Cell Dev Biol · 2022 · PMC8928218 · PMID 35309913

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Unlike for yeast septin rods, adding MgCl 2 to the exchange reaction after 20 min did not change the overall shape of the uptake kinetics but prolonged t 1/2 slightly (and barely significantly) to 14.5 min for hexamers and 21 min for octamers ( Supplementary Figure S5A and Figure 4C ).

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