Quantitative RT-PCR showed an increasing trend in the expression of osteogenic marker genes ( Osterix , Collagen 1a1 , Runx2 , and Osteocalcin ) in MC3T3-E1 cells 10 days after osteogenic induction in the presence of Lef, LFM, and 1-5 ( Figure 3 A).
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Cell-Based Double-Screening Method to Identify a Reliable Candidate for Osteogenesis-Targeting Compounds.
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