Our GWAS of 10 unrelated EOAD-affected dogs and 107 control dogs identified one highly significant region on CFA18 and two other marginally significant regions on CFA24 and CFA26 (Figs 1 and S2 ).
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Early onset adult deafness in the Rhodesian Ridgeback dog is associated with an in-frame deletion in the EPS8L2 gene.
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To evaluate marginally significant associations identified in the main GEMMA analysis, we repeated the analysis with the same number of case and control samples (10 and 107, respectively) out of the entire dataset including related individuals (23 case and 162 control dogs) by randomization with replacement for 100 times.