on the borderline of significancep = 0.062
Cooling cells down enhanced the level of spontaneous foci: the difference in frequency of spontaneous 53BP1 foci in cells cooled to 0.8 °C (3.68 ± 0.96 foci per cell) and those kept at 37 °C (1.59 ± 1.03 foci per cell) was very large (d = 2.1) and on the borderline of significance (p = 0.062).