A Human Conditionally Immortalized Proximal Tubule Epithelial Cell Line as a Novel Model for Studying Senescence and Response to Senolytics.

Regarding β-gal ( Figures 3E,G ), the protein expression levels show an increasing trend at both 33 and 37°C temperatures, there were significant increases of β-gal expression compared to non-senescent cells (Day 0) at Days 3 and 6 at 37°C, with levels being markedly higher at non-permissive compared to permissive conditions at Day 6. 3.4 Maturation of ciPTEC-OAT1 at Non-Permissive Temperature Induces Common SASP Factors Secretion As shown in Figure 4 , the secretion profile of some typical SASP factors (IL-6, TGF-β1, TNF-α, IL-8, and CTGF) clearly correlate with prolonged culture at 37°C, which is indicative of a senescence phenotype.

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The total-p53 levels ( Figures 3A,C ) were markedly upregulated in a time-dependent manner in the proliferation group at 33°C but presented a decreasing trend of expression in the maturation group at 37°C.

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