Morphological characteristics and abundance of prokaryotes associated with gills in mangrove brachyuran crabs living along a tidal gradient.

Counts of prokaryotic cells using SEM imaging, showed a decreasing trend of cell density in the more landward-adapted species ( Fig 5 ). qPCR analysis ( Fig 6 ) corroborates (i) the SEM counting indicating that most of the prokaryotic cells imaged by SEM may belong to the bacteria Domain, and (ii) the significant decrease of bacterial numbers toward the supratidal species.

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