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So, you want to create a frog cell line? A guide to establishing frog skin cell lines from tissue explants.

MethodsX · 2022 · PMC9048074 · PMID 35492210

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If there is not yet significant outgrowth of the tissue explant cultures after 4 weeks of initiation, begin replenishing half of the medium every 7 - 14 days using early passage medium. 22.

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Quoted from the open-access full text in Europe PMC under the licence the publisher applied. The sentence is reproduced exactly as published; the emphasis is ours.