Genome sequencing of Pseudomonas aeruginosa strain M2 illuminates traits of an opportunistic pathogen of burn wounds.

aeruginosa M2 proteins were used as queries in blastp searches against 5 distinct taxonomic databases: (1) “Pseudomonas excluding Pseudomonas aeruginosa ,” (2) “Pseudomonadaceae excluding Pseudomonas,” (3) “Pseudomonadales excluding Pseudomonadaceae,” (4) “Gammaproteobacteria excluding Pseudomonadales,” and (5) “Bacteria excluding Gammaproteobacteria.” The top 200 subjects from each search were merged and ranked by Sm score, a comparative sequence similarity score designed to de-emphasize highly significant matches to short stretches of query (i.e. conserved domains) in favor of longer stretches of similarity ( Driscoll et al. 2013 ).

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