Multiple Methods Synergistically Promote the Synchronization of Somatic Embryogenesis Through Suspension Culture in the New Hybrid Between <i>Pinus elliottii</i> and <i>Pinus caribaea</i>.

Effect of N Form and Concentration on the Synchronization of Somatic Embryos The variance analysis results showed that the concentration of NH 4 + and the interaction between NH 4 + and NO 3 – had an extremely significant effect on SE, but the effect of the concentration of NO 3 – was not significant ( Table 2 ).

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The results showed that concentrations of ABA in the medium in the suspension culture stage had a strong impact on the synchronous maturation of somatic embryos, reaching a highly significant level ( Table 2 ); with the increase of ABA concentration, the highest number of mature cotyledonary embryos were achieved at 37.83 μM/L, with the average number of mature cotyledonary embryos of 274.7, which were induced from 1 ml PCV of cell suspension ( Figure 5A ).

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