These genes showed highly significant differences in round spermatids, and Prm1 was expressed 2.0- and 2.3-fold higher in transplanted fresh or short-frozen, respectively, and 2.8-fold higher in fresh rat as compared with transplanted long-frozen.
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Reestablishment of spermatogenesis after more than 20 years of cryopreservation of rat spermatogonial stem cells reveals an important impact in differentiation capacity.
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