Furthermore, single-molecule RNA fluorescence in situ hybridisation (smRNA FISH) showed a clear and highly significant mutual exclusion between Thbs1-expressing cells and cells showing YAP activation, as assessed by expression of the YAP targets Ctgf ( Figure 5—figure supplement 1H ), Cyr61 ( Figure 5—figure supplement 1I ), Sca1 ( Figure 5C and D , Figure 5—figure supplement 1J ), and indicating the presence of two distinct tumour cell populations: one Thbs1+/Sca1- (64.61% ± 29.14%) and one Thbs1-/Sca1+ (30.40% ± 27.72%) ( Figure 5D ).
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Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programme.
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