We also measured the expression of several more mature PGC markers using qPCR and found that DPPA3 (stella) and DDX4 (vasa) show an increasing trend between 48 and 96 hr, indicating that after their initial specification PGC development continues ( Figure 2F , Figure 2—figure supplement 1E ).
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Efficient differentiation of human primordial germ cells through geometric control reveals a key role for Nodal signaling.
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