Asparagine biosynthesis as a mechanism of increased host lethality induced by <i>Serratia marcescens</i> in simulated microgravity environments.

Gene expression analyses showed that the expression level of the gene asnB (asparagine synthetase B) was 39.17 fold higher in LSMMG-treated cells relative to the RWV-control (P < 0.0001), as well as a 10.5 fold increase in expression of L-asparaginase ( L-ap , P = 0.054), but there was no significant difference in expression for the other genes tested, although several genes showed a positive trend for expression levels: L-ap1 (L-asparaginase 1), L-ap2 (L-asparaginase 2), iaaA (isoaspartyl peptidase/L-asparaginase), or astL (asparagine tRNA ligase) ( Figure 1 D). asnB is a gene that catalyzes the conversion of aspartate to asparagine using ATP and glutamine, and is known to be linked to virulence and antibiotic resistance in bacterial pathogens [ 36 , 37 ].

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