Long-range electrostatic interactions significantly modulate the affinity of dynein for microtubules.

However, the addition of 100 mM KCl had a much greater (a 12-fold increase), highly significant ( p < 0.0001, two-sample t -test, N = 4 and N = 2 for 0 and 100 mM KCl, respectively, Fig. 3 A ) effect on the K d for the D3402A mutant MTBDs than WT MTBDs.

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